19th IBS International Scientific Committee v

19th IBS Local Organizing Committee vi

Foreword vii

Acknowledgements ix

Supporters x

Honouring Douglas E.Rawlings xi

In Memory of Oswaldo Garca Jnior xii

In Memory of Anthony Pinches xiii

In Memory of Geoffrey Spearing Hansford xiv

Section I: Honorary Lecture and Keynote Speeches

Honorary Lecture

Some important developments in biomining during the past thirty years

Douglas E.Rawlings…………………………………………………………………………(3)

Keynote Speeches

The progress of biohydrometallurgy in China

Dianzuo Wang, Guanzhou Qiu, Xueduan Liu…………………………………………………………………………(13)

In situ leaching—How far in the future?

Robin Batterham…………………………………………………………………………(21)

Reductive processes in biohydrometallurgy: New opportunities for mineral processing and for metal recovery from waste streams and process waters

D.Barrie Johnson…………………………………………………………………………(23)

Genes involved in iron and sulfur oxidation pathways in Acidithiobacillus ferrooxidans are regulated by ferrous iron and the global redox responding regBA two component system

Jos Sandoval Ponce, Danielle Moinier, Deborah Byrne, Agns Amouric, Violaine Bonnefoy…………………………………………………………………………(30)

Anaerobic iron bio-reduction: Effectiveness of an alternative hydrometallurgical process

Laura Castro, Camino Garca-Balboa, Felisa Gonzlez, Antonio Ballester, M.Luisa Blzquez, Jess A.Muoz…………………………………………………………………………(37)

New insight in copper resistance mechanism of biomining microorganisms

Carlos A.Jerez…………………………………………………………………………(45)

Interfacial phenomena of bioleaching

Miao Chen…………………………………………………………………………(46)

Mining Terrestrial Metagenomes for Meaning

James M.Tiedje…………………………………………………………………………(47)

Industrial Applications of Bioleaching and Mineral Biooxidation

James A.Brierley…………………………………………………………………………(48)

Acidithiobacillus caldus: A story stats at its genome sequencing

Shuangjiang Liu…………………………………………………………………………(49)

Section II: Fundamentals of Bioleaching

Extended Abstracts

Respirometry studies of the bioleaching of a copper ore in large columns—Effect of changing temperatures

Jochen Petersen, Sanet H.Minaar, Chris A.du Plessis…………………………………………………………………………(53)

Generation of reactive oxygen species from sulfide concentrates as a function of mineralogy

Gavin C.Jones, Robert P.van Hille, Susan T.L.Harrison…………………………………………………………………………(59)

Column bioleaching of low-grade copper ores by Acidithiobacillus ferrooxidans in pure and mixed cultures with a heterotrophic acidophile Acidiphilium sp.

Yu Yang, Mengxue Diao, Kai Liu, Lin Qian, Anh V.Nguyen, Guanzhou Qiu…………………………………………………………………………(66)

Isolation and characterization of manganese solubilising bacteria and its potential for manganese bioleaching

A.P.Das, L.B.Sukla, N.Pradhan, S.Nayak…………………………………………………………………………(73)

Contribution of ferric-iron precipitate to the kinetics of microbial ferrous-iron oxidation by Leptospirillum ferriphillum in a packed column

F.Chowdhury, T.V.Ojumu…………………………………………………………………………(76)

Experiment research and one-dimensional numerical simulation of solute transmission in leaching processes

Jinzhi Liu, Dongmei Huang…………………………………………………………………………(81)

Thermal effect of acidification in heap bioleaching of chalcopyrite

Zhenyi Zhang, Koichi Suto, Chihiro Inoue, Masataka Ohara, Taro Kamiya, Xuan Zhao…………………………………………………………………………(85)

Nickel and cobalt recovery from thermally activated chromite overburden of sukinda mines using fungus mediated bio-acids

S.K.Behera, L.B.Sukla, B.K.Mishra…………………………………………………………………………(89)

Analysis of sulfur speciation on chalcopyrite bioleached with mesophile Acidithiobacillus ferrooxidans

Huan He, Jinlan Xia, Yi Yang, Guanhua Huang, Xiuxiang Tao, Yunwei Leng, Yidong Zhao…………………………………………………………………………(95)

The effect of temperature on the kinetics of ferrous-iron biooxidation by Leptospirillum ferriphilum in a packed column bioreactor

F.Chowdhury, TV Ojumu…………………………………………………………………………(101)

The external appearance of microorganism used to bioleaching copper from waste printed circuit boards under SEM

Zheng Xu, Tao Yang, Limei Yang…………………………………………………………………………(106)

Liquid nitrogen preservation of Acidithiobacillus ferrooxidans using a new cryoprotectant

Xueling Wu, Dongmei Hou, Xiaohong Xin, Bo Miao, Yangyang Wang, Li Shen…………………………………………………………………………(110)

Effect of Cl^- on the electrochemical behavior of chalcopyrite in the bioleaching system

Tian Zhuang, Jun Wang, Wenqing Qin, Yansheng Zhang, Congren Yang, Shaoshi Lai, Laishun Li, Guanzhou Qiu…………………………………………………………………………(114)

Heavy metal resistance of two Acidithiobacillus ferrooxidans strains

Qi Hu, Xueling Wu, Huaqun Yin, Xueduan Liu…………………………………………………………………………(118)

Bioleaching of Cu from enargite using Acidianus brierleyi with concomitant passivation of As

Keiko Sasaki, Koichiro Takatsugi, Tsuyoshi Hirajima…………………………………………………………………………(123)

Research on chemical leaching mechanism of chalcopyrite with difference mineralization

Jing Bai, Jiankang Wen, Songtao Huang, Biao Wu, Guocheng Yao…………………………………………………………………………(126)

The dynamics of chalcopyrite bioleaching in batch and continuous flow reactor systems incorporating time-varying particle surface areas

Athanasios Kotsiopoulos, Geoffrey S.Hansford, Randhir Rawatlal…………………………………………………………………………(129)

Effect of [Fe³⁺]/[Fe²⁺] ratio on sulfides dissolution

Biao Wu, Jiankang Wen, Bowei Chen, Guocheng Yao, Dianzuo Wang…………………………………………………………………………(140)

The mechanism of bioleaching of heavy metals from sewage sludge using Thiobacillus thiooxidans

Heqin Liu, Yeming Wen, Qingping Wang, Zuliang Chen…………………………………………………………………………(145)

Bioleaching of chalcopyrite by mixed cultures of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans

Guohua Gu, Keting Hu, Shuangke Li, Guanzhou Qiu…………………………………………………………………………(150)

Effect of inoculum size on the rates of whole ore colonisation of mesophilic, moderate thermophilic and thermophilic acidophiles

Olga V.Tupikina, Susanna H.Minnaar, George F.Rautenbach, David W.Dew, Susan T.L.Harrison…………………………………………………………………………(156)

Effect of organic extractant, pH and temperature change, on bioleaching microorganisms during SX treatment

Carol S.Davis-Belmar, Ivn Gallardo, Cecilia Demergasso, George Rautenbach…………………………………………………………………………(164)

Parameters influencing the microbial oxidation activity in the industrial bioleaching heap at Escondida mine, Chile

Pamela E.Soto, Pedro A.Galleguillos, Vctor J.Zepeda, Cecilia S.Demergasso, Carlos Pinilla…………………………………………………………………………(169)

Effects of different energy substrates and nickel /cadmium ions on the growth of Acidithiobacillus ferrooxidans

Lei Xiao, Jiongtian Liu, Jinghua Yao, Huan He…………………………………………………………………………(175)

Bioleaching of primary chalcopyrite by A.caldus and S.thermosulfidooxidans

Yuting Liang, Wenqing Qin, Huaqun Yin, Xueduan Liu…………………………………………………………………………(179)

Visualization of flow behavior during bioleaching of waste rock dumps under saturated and unsaturated conditions

Shenghua Yin, Aixiang Wu, Hongjiang Wang, Kaijian Hu…………………………………………………………………………(182)

Bioleaching of chalcopyrite at 40℃ by enrichment culture obtained from some fumarolic areas

Masatoshi Sakoda, Tadashi Chida, Naoki Sato…………………………………………………………………………(190)

The influence of temperature on the bioleaching of copper sulfide ore

E Zhou, Dongping Duan, Siming Chen, Wenyan Liu, Jiankang Wen, Dianzuo Wang…………………………………………………………………………(201)

The effect of KNO₃ on substrate utilisation by Sulfobacillus sp. in mixed ferrous ion and tetrathionate growth medium

D.W.Shiers, D.E.Ralph, H.R.Watling…………………………………………………………………………(204)

Bioleaching behavior of chalcopyrite with carrolite by mesophile and moderate thermophile

Xue Liu, Jiankang Wen, Biao Wu,Shuang Liu, Hong Zang…………………………………………………………………………(211)

Stoichiometric modelling of ferric iron reduction in aerobic Acidithiobacillus thiooxidans DSM 17318 strain licanantay

Roberto A.Bobadilla Fazzini, Pilar Parada…………………………………………………………………………(215)

The effects of pH, anions, cations and ionic strength on tetrathionate oxidation by Acidithiobacillus caldus

G.J.Zhang, D.W.Shiers, H.R.Watling…………………………………………………………………………(220)

The effect of CO₂ availability on the growth, iron oxidation and CO₂ fixation rates of pure cultures of Leptospirillum ferriphilum and Acidithiobacillus ferrooxidans

C.G.Bryan, C.Davis, N.van Wyk, D.Dew, D.Rautenbach, S.T.L.Harrison…………………………………………………………………………(229)

An electrochemical investigation of chalcopyrite paste electrode in the presence of Acidithiobacillus ferrooxidans

Alin Li, Songtao Huang, Jiankang Wen, Biao Wu, Minglin Wu…………………………………………………………………………(238)

Atomic force microscopy and scanning electrochemical microscopy study on bioleaching of CuxS by acidophilic leaching organisms

Xiaocui Zhao, Miao Chen…………………………………………………………………………(244)

Investigation of the bioleaching of coarse sphalerite ore particles by a mixed culture of mesophilic chemolithotrophs in saturated leach reactors

Yousef Ghorbani, Jochen Petersen, Olga V.Tupikina,Susan T.L.Harrison, Megan Becker, Aubrey Mainza, Jean-Paul Franzidis…………………………………………………………………………(249)

Effects of 4-nonylphenol on iron(II) oxidation by Sulfobacillus thermosulfidooxidans

David Collinson, Denis Shiers, Helen Watling…………………………………………………………………………(258)

Research on the microbial shifting of marmatite bioleaching

Zhiguo He, Shuzhen Li, Fengling Gao, Guohua Gu, Yuehua Hu, Guanzhou Qiu…………………………………………………………………………(266)

Effect of pH and temperature on the adsorption of Acidithiobacillus caldus, Leptospirillum ferriphilum and Sulfobacillus thermosulfidooxidans onto pyrite

Lisha Wang, Jianhua Qu, Yuehua Hu, Zhiguo He, Guohua Gu…………………………………………………………………………(274)

Insights into the effect of external species introduction on pyrite bioleaching

Jie Liu, Xiangru Fan, Yi Liu, Xueduan Liu, Guanzhou Qiu, Qian Li, Yili Liang, Xian Fu, Huaqun Yin…………………………………………………………………………(280)

Extraction of ferric iron from mineral surface and its function during bioleaching of chalcopyrite

Weimin Zeng, Guanzhou Qiu, Miao Chen…………………………………………………………………………(287)

Synergic action of Fe and bacteria in gold bearing chalcopyrite bioleaching

Pengcheng Ma, Hongying Yang, Zhanqi Han…………………………………………………………………………(293)

Short Abstracts

The influence of jarosite on bioleaching of chalcopyrite and pyrite

Rongbo Shu, Yachuan Liu, Xiuxiang Tao, Jiongtian Liu…………………………………………………………………………(298)

Stable isotopes—What do they tell us about bioleaching mechanisms?

Axel Schippers…………………………………………………………………………(299)

CSIRO heap bioleaching model: Current status and future trends

Pablo Dupuy, Koji Akama, Martin Leahy, Phil Schwarz…………………………………………………………………………(300)

Microbial population dynamics on low grade whole ore containing chalcopyrite during temperature succession from 25℃ to 65℃

Olga V.Tupikina, S.Minnaar, R.P.van Hille, N.van Wyk, D.Rautenbach, D.Dew, Susan T.L.Harrison…………………………………………………………………………(301)

Integrated spatial and temporal kinetic of chalcosite in bioheap leaching: A biogeometallurgical road map

Patio, Eduardo.Thomas, Mark. Mendoza, Gonzalo.Batallanos, Karim.…………………………………………………………………………(302)

The effect of chloride on the electrochemical potential during the bioleaching of pure chalcopyrite and low grade copper sulfide ores

Zepeda V., Demergass C., Blquez M.L., Muoz A., Gonzlez F., Ballester A.…………………………………………………………………………(303)

Functional genomics analysis of the extreme thermoacidophile Metallosphaera sedula provides insights into the archaeons unusual growth physiology

Hong Lian, Aaron B.Hawkins, Arpan Muhkerjee, Kathryne S.Auernik, Yejun Han, Robert M.Kelly…………………………………………………………………………(304)

Bioleaching of indium from sulfide ore

Chihiro Inoue, Jun Sato, Yasumasa Ogawa, Yui Takahashi, Roichi Yamada, Koichi Suto…………………………………………………………………………(305)

Ferrous iron oxidation by a Leptospirillum ferriphilum dominated culture under electrochemically controlled redox potentials

Agnieszka Pawlowska, Chandra Sekhar Gahan, Jan-Eric Sundkvist, ke Sandstrm…………………………………………………………………………(306)

The study of the effect of stirring speed, air rate and pulp density of sphalerite on the overall volumetric oxygen transfer coefficient (k_La) in a mechanically agitated bioreactor using factorial design of experiments

Negar Basereh Taromsary, Babak Bonak darpour, Eskandar Keshavarz Alamdari…………………………………………………………………………(307)

Kinetics of sodium jarosite precipitation in the system Fe₂(SO₄)₃-H₂SO₄-NaOH at 70℃

Carolina Paipa, Mara Ins Toral, Marcelo Campos-Vallette, Toms Vargas…………………………………………………………………………(308)

Bioleaching mechanism of orpiment by Thiobacillus thiooxidans and Thiobacillus ferrooxidans without additional iron

Xingwu Chao,Guangji Zhang, Zhaoheng Fang, Zhichang Liu, Chao Yang…………………………………………………………………………(309)

A gradually-shrunk draft tube for improving suspension of solid particles in a stirred bioreactor

Gang Deng, Xiangyang Li, Chao Yang, Zaisha Mao, Guangji Zhang, Xiaoheng Fu…………………………………………………………………………(310)

Solid suspension and gas dispersion performance in a dual-impeller stirred tank bioreactor

Hongbao Zhou, Xiangyang Li, Guangji Zhang, Chao Yang, Zaisha Mao…………………………………………………………………………(311)

Electrocatalytic effects of pyrite on copper bioleaching sulfide minerals

M.Schaffie, A.Ahmadi, M.Ranjbar…………………………………………………………………………(312)

In situ EC synchrotron XRD study transformation of the intermediate product on sulfide mineral during bioleaching

Miao Chen, Yansheng Zhang, Weimin Zeng, Nathan A.S.Webster, Ian C Madsen…………………………………………………………………………(313)

Microbial leaching behaviour of pyrite

Su Nee Tan, Miao Chen…………………………………………………………………………(314)

Comparison studies of bioleaching functions of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans on pyrite by using scanning electrochemical microscopy

Xiaocui Zhao, Miao Chen…………………………………………………………………………(315)

Investigation of the nature of the passivating layer in galvanically assisted chalcopyrite leaching

Seyed Mohammad Khoshkhoo Sany, Valeh Aghazadeh, Andrey Shchukarev, ke Sandstrm, Mark Dopson…………………………………………………………………………(316)

Screening and characterization of sulfur activation proteins of Acidithiobacillus ferrooxidans ATCC23270

Ruiyong Zhang, Jinlan Xia, Anan Peng, Chenggui Zhang, Zhenyuan Nie, Guanzhou Qiu, Wolfgang Sand…………………………………………………………………………(317)

Spectroscopic study on role of Achidithiobacillus caldus in bioleaching of chalcopyrite

Keiko Sasaki, Koichiro Takatsugi, Naoko Okibe, Tsuyoshi Hirajima, Olli Tuovinen…………………………………………………………………………(318)

Section Ⅲ: Genetics and Molecular Biology

Extended Abstracts

Genomic islands and their importance in metal resistance for biomining bacteria

Luis H.Orellana, Claudio A.Navarro, Carlos A.Jerez…………………………………………………………………………(321)

Codon usage analysis of Acidithiobacillus ferrooxidans: Extreme GC-preference on third base

Hong Peng, Anh Nguyen, Xueduan Liu, Yu Yang, Yuehua Hu…………………………………………………………………………(327)

C-di-GMP pathway in the biomining bacteria Acidithiobacillus thiooxidans

M.Diaz, V.Nicoli, M.Castro, D.Holmes, N.Guiliani…………………………………………………………………………(336)

Grand challenges for bioleaching

Yuandong Liu,Guanzhou Qiu, Weimin Zeng…………………………………………………………………………(341)

Identification and functional analysis of a regulatory protein for the pet II operon in Acidithiobacillus ferrooxidans ATCC23270

Yu Yang, Jiangjiang Li, Xiang Wang, Li Yang, Zhiying Huang, Xueduan Liu, Guanzhou Qiu…………………………………………………………………………(344)

Mechanisms of protein stabilization at very low pH

Francisco Duarte, Rene Sepulveda, Raul Araya, Sebastian Flores, Tomas Perez-Acle, Wendy Gonzales, Danilo Gonzales, Goran Neshich, Izabella Neshich, Ivan Mazoni, David S.Holmes…………………………………………………………………………(349)

Comparative study of redox stress response in the acidophilic bacteria Leptospirillum ferriphilum and Acidithiobacillus ferrooxidans

Andrs Corts, Rodrigo Flores, Javiera Norambuena, Juan Pablo Crdenas, Raquel Quatrini,

Renato Chvez, Omar Orellana, Gloria Levicn…………………………………………………………………………(354)

High throughput proteomic comparison of data for planktonic and biofilm-forming cells of Acidthiobacillus ferrooxidans ATCC 23270

Mario Vera, Beate Krok, Wolfgang Sand, Ansgar Poetsch…………………………………………………………………………(358)

Transcriptomic studies of capsular polysaccharide export systems involved in biofilm formation by Acidithiobacillus ferrooxidans

Sren Bellenberg, Mario Vera, Wolfgang Sand…………………………………………………………………………(362)

Expression and characterization of an Acidithiobacillus ferrooxidans ATCC23270 heat shock protein hsp90 (AtHtpG) functionally expressed in Escherichia coli

Jianyu Zhu, Qiuxia Huang, Weifeng Jiao, Qian Li, Yuehua Hu, Wenqing Qin, Xueduan Liu, Guanzhou Qiu…………………………………………………………………………(366)

¹³C NMR study on glucose metabolic pathways of genetic engineered chemolithotrophic Acidithiobacillus thiooxidans expressing phosphofructokinase

Wei Liu, Jianqun Lin, Xiangmei Liu, Jianqiang Lin…………………………………………………………………………(373)

Characterization of apparent sulfur oxidation activity of thermophiles in bioleaching of chalcopyrite

Wei Zhu, Jinlan Xia, Anan Peng, Zhenyuan Nie, Guanzhou Qiu…………………………………………………………………………(378)

Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859

Wei Liu, Jianqun Lin, Xin Pang, Shuang Mi, Shuang Cui, Jianqiang Lin…………………………………………………………………………(383)

Copper resistance determinants of the biomining thermoacidophilic archaeon Sulfolobus metallicus

Francisco Remonsellez, Alvaro Orell, Carlos A.Jerez…………………………………………………………………………(388)

Structure prediction and docking study of RpiA from extremophile Acidithiobacillus ferrooxidans

Yuandong Liu,Guanzhou Qiu…………………………………………………………………………(393)

Bioinformatic prediction and experimental verification of sulfate assimilation genes in the extreme acidophile Acidithiobacillus ferrooxidans

Chunli Zheng, Jianshe Liu, Min Wang, Yunjie Dai…………………………………………………………………………(400)

Short Abstracts

Challenges for life at pH 1: Terrestrial evolution and extra-terrestrial life signatures

David S.Holmes…………………………………………………………………………(404)

Crystal structure and mechanism of sulfide: Quinone oxidoreductase from Acidithiobacillus ferrooxidans

Yanfei Zhang, Maia M.Cherney, Joel.H.Weiner…………………………………………………………………………(405)

The complete genome of the crenarchaeon Metallosphaera cuprina

Chengying Jiang, Lijun Liu, Xiaoyou Yan, Shengyue Wang, Shuangjiang Liu…………………………………………………………………………(406)

Analysis of a protein involved in the regulation of sulfur and iron oxidation by Acidithiobacillus ferrooxidans

Kazuo Kamimura, Mei Kikumoto, Jun Takada, Tadayoshi Kanao…………………………………………………………………………(407)

Section IV: Microbe-Mineral Interactions

Extended Abstracts

Comparative study of pyrite and chalcopyrite bioleaching by mesophilic strains of mine Tizapa (Mexico)

R.E.Rivera-Santilln, M.E.Cruz-Snchez, S.T.Camargo-Villafuerte…………………………………………………………………………(411)

Adsorption characteristics and mechanism of biosolubilization of rock phosphate by Aspergillus niger

Chunqiao Xiao, Ruan Chi, Yujuan Fang…………………………………………………………………………(417)

Effect of ore particle size and mixed culture on fungal bioleaching

Karthik Shet, Vidya Shetty K…………………………………………………………………………(423)

Effect of free and attached moderate thermophilic microorganisms on metal solubilization in bioleaching system for zinc smelting slag under different pH

Kaiqi Jiang, Zhaohui Guo, Yi Cheng, Long Zhang, Xiyuan Xiao…………………………………………………………………………(428)

Evolvement of surface property of extracellular polymeric substances of At.ferrooxidans with culture media and time

Runlan Yu, Daili Zhong, Fadeng Wu, Zhijun Peng, Jiaju Ji, Wenqing Qin, Guanzhou Qiu…………………………………………………………………………(434)

Flocculation of quartz by microorganisms

Tsuyoshi Hirajima, Yuki Aiba, Mohsen Farahat, Naoko Okibe, Keiko Sasaki, Takehiko Tsuruta, Katsumi Doi…………………………………………………………………………(441)

Fungal biotransformation of anthracite-grade carbonaceous matter: Effect on gold cyanide uptake

Grace Ofori-Sarpong, Kwadwo Osseo-Asare, Ming Tien…………………………………………………………………………(445)

Electrostatic contributions in the adhesion of Escherichia coli and Bacillus subtilis onto gold substrate in aqueous environment

Jose Carlo M.Viguilla, Windell L.Rivera, Herman D.Mendoza…………………………………………………………………………(452)

A lectin-based approach for visualization of capsular EPS after attachment and biofilm formation by acidophilic leaching bacteria

Sren Bellenberg, Felipe Leon-Morales, David Holuscha, Beate Krok, Mario Vera, Wolfgang Sand…………………………………………………………………………(460)

Surface passivation of chalcopyrite in bioleaching process

Guohua Gu, Keting Hu, Yuwu Guo, Guanzhou Qiu…………………………………………………………………………(465)

Quorum sensing in Acidithiobacillus ferrooxidans ATCC 23270

S.Mamani, Y.Denis, L.Soulre, A.Doutheau, Y.Queneau, C.Demergasso, V.Bonnefoy, N.Guiliani…………………………………………………………………………(472)

Separation and analysis of lignite bioconversion products

Jinghua Yao, Xianyong Wei, Lei Xiao…………………………………………………………………………(476)

Influences of initial Fe(II) and monovalent cation concentrations on the formation of biogenic iron hydroxysulfate precipitates in acidic sulfate-rich environments

Shuangyou Bai, Min Wang, Jianru Liang, Lixiang Zhou…………………………………………………………………………(484)

Uncharted biotechnologies for gold exploration and processing

Carla Zammit, Davide Quaranta, Nigel Cook, Joёl Brugger, Frank Reith…………………………………………………………………………(491)

Microbial activity in the schwertmannite formed in a mine-water treatment plant

Judith Kipry, Claudia Wiacek, Michael Schlmann…………………………………………………………………………(499)

The surface behavior of Rhodococcus opacus on calcite, serpentine and dolomite

Hongxu Li, Chuanqi Jiao, An Li, Binin Liu…………………………………………………………………………(502)

The behaviour of silver, its chemical compounds and arsenic during biohydrometallurgical treatment of refractory concentrates

Olga D.Kheml, nitskaya…………………………………………………………………………(510)

Short Abstracts

Perspectives of surface nanomorphology on biochemical activity of metal oxide, sulphide and silicate nanoparticles with reference to minerals bioprocessing

K.Hanumantha Rao…………………………………………………………………………(515)

Geomicrobiological investigation of manganese nodules collected during the R/V Sonne cruise SO 205 from the Clarion-Clipperton-Zone, Pacific Ocean

Marco Blthe, Axel Schippers…………………………………………………………………………(516)

Surface modification by microorganisms in froth flotation

S.Komar Kawatra, Timothy C.Eisele…………………………………………………………………………(517)

Influence of solution conditions on sulphur oxidizing bacterial yields and activity

Rachel Candy, Kyle Blight, David Ralph…………………………………………………………………………(518)

Characterization of bio-minerals after enzymatic oxidation of Mn(II) using a Mn-oxidizing fungus in the presence of Zn²⁺

Qianqian Yu, Keiko Sasaki, Tsuyoshi Hirajima…………………………………………………………………………(519)

Anodic oxidation of pyrite in the presence of Acidithiobacillus ferrooxidans by alternating current impedance technique

Shaofen Wang, Li Xiao, Zheng Fang, Guanzhou Qiu…………………………………………………………………………(520)

Mechanism of bio-leaching of chalcopyrite in presence of Acidithiobacillus ferrooxidans

Li Xiao, Guanzhou Qiu, Zheng Fang, Shaofen Wang…………………………………………………………………………(521)

Electrochemical behavior of anilite in presence of Acidithiobacillus ferrooxidans

Haina Cheng, Yuehua Hu…………………………………………………………………………(522)

Getting into the sulfur speciation transformation during bioleaching of sulfide minerals and sulfur oxidation by sulfur-oxidizing bacteria is a peculiar useful way to elucidate mineral dissolution and sulfur oxidation mechanisms

Jinlan Xia, Chenggui Zhang, Huan He, Yi Yang, Changli Liang, Wei Zhu, Xiaojuan Zhao, Ruiyong Zhang, Anan Peng, Zhenyuan Nie, Yidong Zhao, Guanzhou Qiu…………………………………………………………………………(523)

Interactions of indigenous bacteria with organic-rich Kupferschiefer black shale from the Lubin-Sieroszowice area (Fore-Sudetic Monocline, Poland)

Renata Matlakowska, Aleksandra Sklodowska, Krzysztof Nejbert…………………………………………………………………………(525)

NanoSIMS investigations into the utilization of iron by bioleaching microorganisms growing on chalcopyrite

Kayley M.Usher, Anna H.Kaksonen, Matt Kilburn, John Cliff…………………………………………………………………………(526)

The Carnouls mine (Gard, France) geomicrobiological characterization of an As-rich acid mine drainage, a ten year study

Bruneel O., Casiot C., Lauga B., Arsene-Ploetze F., Fahy A., Goi-Urriza M.S., Morin G., Personn J-C., Bertin P.N., Duran R., Elbaz-Poulichet F.…………………………………………………………………………(527)

Patterns of adherence of Sulfolobus metallicus on chalcopyrite and their relation with the sulphide dissolution mechanism

Toms Vargas, Vernica Gautier, Carol Davis, Helmut Tributch…………………………………………………………………………(528)

Comparison of attachment and growing dynamics of two Acidithiobacillus ferrooxidans strains exposed to four pure sulfide minerals

Alex Echeverra, Cecilia Demergasso…………………………………………………………………………(529)

Challenges and opportunities for use of microorganisms in minerals processing

S.Komar Kawatra, Timothy C.Eisele…………………………………………………………………………(530)

Section V: Bioleaching Applications

Extended Abstracts

Inoculation of chemolithotrophic bacteria into heaps consisting of a freshly excavated copper mixed ore

Veneta Groudeva, Dimiter Vasilev, Stoyan Groudev…………………………………………………………………………(533)

Gold nanoparticles production using algae and industrial waste biomass

Laura Castro, M.Luisa Blzquez, Antonio Ballester, Jess A.Muoz, Felisa Gonzlez, Camino Garca-Balboa…………………………………………………………………………(540)

Bioleaching of copper from waste electronic scraps by cultures of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans

Jinqiu Xu, Jianfeng Bai, Jingwei Wang, Bo Liang, Jie Guan…………………………………………………………………………(547)

Bio-oxidation pretreatment of a sulfide concentrate including gold to improve the utilization of valuable elements

Meilin Liu, Jiankang Wen, Yongsheng Song…………………………………………………………………………(555)

Current situation and development of biooxidation of refractory gold ores in China

Yujie Guo, Xinxing Liu, Dianzuo Wang…………………………………………………………………………(560)

Study on causes of interfacial emulsification in solvent extraction of copper

Guiying Zhou, Jiankang Wen…………………………………………………………………………(562)

Bioleaching of enargite dominant copper sulfide under mesophilic and thermophilic conditions: Copper and arsenic extractions

Jaeheon Lee, Sevket Acar, James A.Brierley…………………………………………………………………………(565)

A comprehensive study on separation of copper from complex sulphide ore bioleaching solution

Wei Luo, Dianzuo Wang, Jiankang Wen, Yongsheng Song, Guocheng Yao…………………………………………………………………………(574)

Influence of sulfur source on nickel and cobalt bioleaching from laterite tailings using Acidithiobacillus thiooxidans

Orquidea Coto, Axel Schippers…………………………………………………………………………(579)

Material size distribution in concurrent bio-leaching and precipitation: Experimental procedure and modelling

Pierre Botane, Stephane Brochot, Patrick DHugues, Pauline Spolaore…………………………………………………………………………(584)

Bioleaching of whitehorse copper tailings with use of tank method

Muyassar G.Sagdieva, Sergey I.Borminskiy, Aziza M.Mavjudova, G.V.Cherkasova, Brian Scott…………………………………………………………………………(593)

Biooxidation of refractory gold bearing ores by moderately thermophilic chemolithotrophic bacteria and their association

Narine Vardanyan, Levon Markosyan, Arevik Vardanyan…………………………………………………………………………(597)

An industrial heap bioleaching of fluorine-bearing uranium ore

Xuegang Wang, Jiang Li, Zhanxue Sun, Yajie Liu…………………………………………………………………………(601)

Microorganisms for nickel laterite leaching

Ekaterina A.Kim,Lyubov N.Krylova, Eduard V.Adamov…………………………………………………………………………(604)

The usage of immobilized Acidithiobacillus ferrooxidans cells for stirred tank leaching of non-ferrous metals

Maxim S.Gusakov, Lyubov N.Krulova, Eduard V.Adamov…………………………………………………………………………(609)

The analysis of biochemical stirred-tank sulfidic concentrate leaching technologies

Eduard V.Adamov, Lyubov N.Krylova, Ekaterina A.Kim…………………………………………………………………………(611)

Jarosite and gypsum impacting on bioleaching of copper and nickel sulfide ores

Congren Yang, Wenqing Qin, Shaoshi Lai, Jun Wang, Yansheng Zhang…………………………………………………………………………(615)

Hydronium jarosite formation and iron balance in bioheapleaching of Zijinshan copper sulphide

Renman Ruan, Gang Zou, Tao Xu, Xingyu Liu, Shuiping Zhong, Xiuzhu Su…………………………………………………………………………(622)

The treatment of gold-bearing refractory concentrates using biooxidation

Luidmila Ye.Shketova, Yuri Ye.Emelianov, Natalia V.Kopylova…………………………………………………………………………(629)

Chalcopyrite bioleaching by mesophilic microorganisms and surface passivation

Guanghua Ai, Yanqiong Tu, Xiuxiang Tao…………………………………………………………………………(632)

A study of alkalophilic bacteria enhencing leaching of copper tailings

Aixiang Wu, Shaoyong Wang, Hongjiang Wang…………………………………………………………………………(638)

The development of technological schemes for tank bio-hydrometallurgical process of refractory gold-bearing sulfide materials

Vigandt K.A., Adamov E.V.…………………………………………………………………………(643)

Research on the bioremediation technology of heavy metal contamination in metallic mines soils

Zhongkui Zhou, Zhanxue Sun…………………………………………………………………………(645)

Isolation and identification of four strains of Leptospirillum ferriphilum from four acid mine drainage sites and their roles in bioleaching of pyrite

Yi Liu, Huaqun Yin, Yao Liu, Qiaoyi Tan, Yili Liang, Xueduan Liu…………………………………………………………………………(651)

Preliminary study on the bioleaching behavior of a high acid-consuming pentlandite/pyrrhotite ore 1: Biotic vs abiotic leaching

Alexandra Muoz, Denise Bevilaqua, Oswaldo Garcia Jr, Wolfgang Sand…………………………………………………………………………(656)

Preliminary study on the bioleaching behavior of a high acid-consuming pentlandite/pyrrhotite ore 2: Pure vs mixed culture

Alexandra Muoz, Denise Bevilaqua, Oswaldo Garcia Jr.…………………………………………………………………………(660)

Effect of density of pulp and size of particle on biooxidation of a polymetallic concentration in the southern sector of Ecuador

P.Aguirre, L.Yaguana, J.C. Gentina…………………………………………………………………………(664)

Fungal bio-leaching of nickel from Indonesian low grade limonitic ore

Mubarok M.Z., Astuti W., Chaerun S.K.…………………………………………………………………………(671)

Determination of technological indicators of sulfidic concentrate biooxidation in the cascade of bioreactors considering bioleaching kinetic

Eduard V.Adamov, Lyubov N.Krylova…………………………………………………………………………(677)

Column bioleaching of apatite rich low grade Indian uranium ore

Abhilash, B.D.Pandey, L.Ray…………………………………………………………………………(680)

Bioleaching of low-grade tellurium ore by mixed bacteria and A.ferrooxidans

Hongguan Xie, Lingfei Lei…………………………………………………………………………(684)

Laboratory investigation on the effect of biodesulphurization process on the sulphide ore self-heating

Shenghua Yin, Aixiang Wu, Hongjiang Wang…………………………………………………………………………(688)

Heterotrophic bioleaching of heavy metal-polluted soil by using Aspergillus niger

Di Fang, Jie Li…………………………………………………………………………(694)

Advancements in the approach to research and design of heap bioleaching processes

Mariekie Gericke, Ali Seyedbagheri, John Neale, Petrus van Staden…………………………………………………………………………(698)

Mercury impact on bioleaching microbial populations in laboratory and industrial like environments

Yasna Contador, Karen Sez, Pedro Galleguillos, Cecilia Demergasso…………………………………………………………………………(706)

Effect of SX on microbial populations inhabiting in solutions of a heap bioleaching process

Cecilia Demergasso, Vctor Iturriaga, Pedro A.Galleguillos, Sergio Davis…………………………………………………………………………(712)

The progress of study on the in-situ bioleaching in a sand stone uranium mine, China

Jinhui Liu, Yipeng Zhou, Yajie Liu, Zhanxue Sun, Weijun Shi…………………………………………………………………………(718)

Influence of microorganisms on arsenic-bearing materials dissolution

Vladimir Luganov, Tatyana Chepushtanova, Erkebulan Kilibayev, Shanibai Baiysbekov…………………………………………………………………………(721)

Copper extraction from primary ore using Acidithiobacillus spp. and Leptospirillum ferrooxidans microorganisms

Dbora M.Oliveira, Luis G.S.Sobral, Carlos E.G.Souza, Gabriel H.C.Peixoto, Eliana F.C.Srvulo…………………………………………………………………………(727)

Pilot scale bioleaching of copper sulphide flotation concentrate using different consortium of mesophile, moderate and extreme thermophile microorganisms

Luis Sobral, Carlos Souza, Dbora Oliveira…………………………………………………………………………(731)

Bioleaching of pyrite gravity concentrate from a coal mine to produce iron oxide pigments

Carlos E.G.de Souza, Dbora M.de Oliveira, Luis G.S.Sobral…………………………………………………………………………(736)

Comparative bioleaching of chalcopyrite concentrate with mesophiles and moderate thermophiles in the stirred tank reactor and their ecology analysis

Weimin Zeng, Guanzhou Qiu, Miao Chen, Hongbo Zhou, Xueduan Liu…………………………………………………………………………(742)

Bioleaching of noble metals from refractory raw materials

Galina Semenchenko, Ayim Mukusheva, Nadezhda Rubanuyk…………………………………………………………………………(749)

New biochemical technology of gold leaching from sulfide ores

Aigul Koizhanova, Larisa Osipowskaja…………………………………………………………………………(756)

Industrialized heap bioleaching research on the secondary sulphide ore in Yulong copper mine

Minglin Wu, Alin Li, Yihanna Hu, Xingxun Zhang, Yong Jiang, Hong Zang, Biao Wu, Jiankang Wen…………………………………………………………………………(760)

Bioleaching of copper sulphide sludge

Milena Cvetkovska, Vladimir Cvetkovski, Milovan Vukovi…………………………………………………………………………(764)

Bioleaching of copper smelter slag

Milena Cvetkovska, Vladimir Cvetkovski, Milovan Vukovi…………………………………………………………………………(770)

Bioleaching of metals from electronic scrap and its potential for commercial exploitation

Sadia Ilyas, Ruan Chi…………………………………………………………………………(774)

Isolation and identification of a mesophilic iron oxidizing bacteria from a Zambias AMD

Ngom Baba, Yi Liu, Huaqun Yin, Yili Liang, Xueduan Liu, Guanzhou Qiu…………………………………………………………………………(782)

Kinetics and modeling of chemical leaching of sphalerite concentrate with ferric iron in redox-controlled reactor

Jian Song, Jianqun Lin, Jianqiang Lin…………………………………………………………………………(789)

Bioleaching of Cu-Zn flotation tailings

Linlin Tong, Hongying Yang, Chunming Liu…………………………………………………………………………(793)

Desulphurizing from vanadium-bearing titanomagnetite concentrates by Acidithiobacillus thiooxidans

Xiaorong Liu, Aiying Chen, Yongsheng Li, Chang Su,Yaqin Sun…………………………………………………………………………(798)

Leaching behavior of secondary zinc oxide dusts in ammonia solutions

Yongbin Yang, Wenjuan Wang, Qian Li, Tao Jiang, Xianju Shi…………………………………………………………………………(803)

Research on control of pH in solvent extraction of zinc by D2EHPA (P204)

Yongbin Yang, Xianju Shi, Qian Li, Tao Jiang, Wenjuan Wang, Hongguang Nie…………………………………………………………………………(808)

The study on heap bioleaching for gold recovery from refractory ores using non-cyanide lixiviant

Sergey S.Gudkov, Yuri Ye. Yemelianov, Liudmila Ye. Shketova, Alexandra N.Mikhailova…………………………………………………………………………(813)

Irgiredmet experience on gold biometallurgy

V.E.Dementyev, G.I.Voiloshnikov…………………………………………………………………………(818)

Bacterial leaching research of Cu-Ni flotation tailing

Jinghe Zhu…………………………………………………………………………(821)

Short Abstracts

Biohydrometallurgical treatment of high clay refractory gold ores—Biooxidation, mineralogy and cyanidation

Jaeheon Lee, Sevket Acar, James A.Brierley…………………………………………………………………………(824)

Bacterial leaching of limonite and saprolite mineral ores by mixotrophic bacterial consortia: Lateritic nickel ore deposits in South Sulawesi, Indonesia

Siti Khodijah Chaerun, Siti Aisyah Alting…………………………………………………………………………(825)

Heap bioleaching of a low grade nickel-cobalt sulfide ore

Hong Zang, Jiankang Wen…………………………………………………………………………(826)

Leaching behavior of calcium from a low grade copper sulfide ore

Congren Yang, Wenqing Qin, Shaoshi Lai, Jun Wang, Yansheng Zhang…………………………………………………………………………(827)

Electrochemical behavior of massive chaclopyrite bioleached electrodes in presence of Sulfobacillus thermosulfidooxidans and Acidithiobacillus ferrooxidans

Wenqing Qin, Shaoshi Lai, Bo Zhang, Jun Wang, Congren Yang, Haohua Kuang, Zhi Mei, Bingchao L, Qiang Cai…………………………………………………………………………(828)

Influence of mechano-chemical activation on bio-oxidation of auriferous sulphides

J.J.K Gordon, E.K.Asiam…………………………………………………………………………(829)

Microbial leaching of nickel from Pomalaa saprolite ores using fungal consortia

Astuti W., Mubarok M.Z, Chaerun S.K…………………………………………………………………………(830)

Processing of noncondition ores at Almalyk mining metallurgical complex using heap bloleaching method

Sagdieva M.G., Borminskiy S.I., Mavjudova A.M., Cherkasova G.V.…………………………………………………………………………(831)

The “siliceous bacteria” as biological agents that break down silicate and possibility of their multipurpose use and application—Some of our laboratory results

Vladimir P.Bekoski, Gordana Gojgi-Cvijovi, Olga Cvetkovi, Vesna Dragutinovi, Sneana Spasi, Dragomir Vitorovi, Miroslav M.Vrvi…………………………………………………………………………(832)

Application prospect of bioleaching in the field of raw ceramic materials purification in China

Lianxiang Yu,Guanzhou Qiu, Haidong Wang, Miao Chen…………………………………………………………………………(833)

Influence of pyrite addition on the electrochemical bioleaching of chalcopyrite concentrates

Ali Ahmadi, Mohammad Ranjbar, Mahin Schaffie…………………………………………………………………………(834)

Fuzzy logic modeling of bioleaching for copper recovery from a flotation copper concentrate

Ali Ahmadi, Maryam Khezri…………………………………………………………………………(835)

Bioleaching of a low-grade sulfide ore mediated by native acidophilic consortium from geothermal Copahue-Caviahue system

P.Chiacchiarini, R.Ulloa, L.Lavalle, A.Giaveno, E.Donati…………………………………………………………………………(836)

Would inoculation of the ore be of any benefit: A methodology to assess in advance the potential impact of bacterial inoculation on copper bioleaching processes

Toms Vargas, Luis Lufin, Carolina Salhe…………………………………………………………………………(837)

Influence of anodic and cathodic sub-processes on the rate of copper dissolution during ferric leaching of chalcopyrite at 70℃

Hctor Jordan, Toms Vargas…………………………………………………………………………(838)

Effects of immobilization on activity of bacteria

Yuanyuan Li, Guangji Zhang, Junya Cao, Zhaoheng Fang, Qiang Xie, Chao Yang…………………………………………………………………………(839)

Iron removal from silica using hot agitated acidic leaching under reducing conditions

Hamed Haghi, Mohammad Noaparast…………………………………………………………………………(840)

Influence of different acid pre-leaching levels on uranium bioleaching performance in column reactors

Qian Li, Huaqun Yin, Miao Chen, Runlan Yu, Zhanxue Sun, Yajie Liu, Yage Zhang, Guanzhou Qiu, Xueduan Liu…………………………………………………………………………(841)

The effect of increasing temperature on the bioleaching of Yinshan low grade complex sulphide ores

Xinxing Liu, Guohua Wang, Yonghong Wu, Shoupeng Li, Yujie Guo, Wanjie Liang…………………………………………………………………………(842)

Microbial extraction of aluminum from black shale using organic acids produced by acidophilic heterotrophs

Muhammad Shahid, Fozia Anjum…………………………………………………………………………(843)

Analysis of microbial population dynamics during bio-pretreatment of refractory arsenical gold ore

Yujie Guo, Xinxing Liu, Yili Liang, Xiumei Wang, Kai Liu, Chenbin Ai, Guohua Wang,

Yonghong Wu…………………………………………………………………………(844)

Thermophilic and mesophilic leaching of copper sulfide sludge

Milena Cvetkovska, Vladimir Cvetkovski…………………………………………………………………………(845)

Sequential evaluation of the effect of iron precipitates, elemental sulfur, and low redox potentials on the passivation of chalcopyrite

Gianfranco Debernardi, Pablo Albistur, Gino Slanzi, Fernando Acevedo, Juan Carlos Gentina…………………………………………………………………………(846)

Dump bioleaching of Zambian Chambishi low grade copper ore

Jun Wang, Wenqing Qin, Guanzhou Qiu, Jinghe Zhu, Xueduan Liu, Hongbo Zhou, Peiwen Zhang, Wenyan Xu, Huaqun Yin, Yansheng Zhang, Weimin Zeng, Tian Zhuang…………………………………………………………………………(847)

Column bioleaching of Zambian Chambishi low grade copper ore

Jun Wang, Guanzhou Qiu, Wenqing Qin, Caoming Huang, Xueduan Liu, Hongbo Zhou, Peiwen Zhang, Yaoling Li, Huaqun Yin, Yansheng Zhang, Weimin Zeng, Laishun Li…………………………………………………………………………(848)

Section VI: Bioflotation and Biobeneficiation

Extended Abstracts

Separation of pyrite and arsenopyrite by bioflotation

Xudong Zhai, Zheng Xu, Limei Yang…………………………………………………………………………(851)

Microbially induced flotation of galena and quartz from pyrite

H.Sarvamangala, K.A.Natarajan…………………………………………………………………………(855)

Beneficiation of high grade copper ore through mineral-microbe interaction

Supriya Sarkar…………………………………………………………………………(861)

Surface morphology of malachite in the process of sulfidization

Dianwen Liu, Jianjun Fang, Xiaolin Zhang, Shuming Wen, Wenbin Zhang…………………………………………………………………………(865)

Bioleaching of flotation chalcopyrite concentrate by a moderately thermophilic enrichment

Yuguang Wang, Hongbo Zhou, Lijuan Zhang…………………………………………………………………………(871)

Short Abstracts

Role of microorganisms in bio-flotation of iron oxide-silica-phosphorus system

Nagui A.Abdel-Khalek, K.Hanumantha Rao, Khaled E.Yassin, Samah S.Abdallah, Khaled A.Selim, Hoda H.El-Hendawy…………………………………………………………………………(878)

Microbially-induced iron ore beneficiation

K.A.Natarajan…………………………………………………………………………(879)

Streptomyces lunalinharesii strain for the biosorptive remediation of copper ions

Diego M.Veneu, Mauricio L.Torem, Gabriela A.H.Pino…………………………………………………………………………(880)

Bioflotation of coal in double discharge flotation cell using taguchi method

Azam Ghadyani, Hamed Haghi, Mohammad Kolahdoozan, Mohammad Noaparast…………………………………………………………………………(881)

Beneficiation of high iron containing damanjodi bauxite using organic acids

Mousumi Mishra, Subhadra Mohanty, Rama Chandra Mohanty, LalaBehari Sukla, BaradaKanta Mishra…………………………………………………………………………(882)

Biomodulation of mineral surfaces for selective separation: An FTIR and SEM study

K.A Natarajan…………………………………………………………………………(883)

Section VII: Biosorption and Bioaccumulation

Extended Abstracts

Comparative analysis of biosorption of lead by pelleted and filamentous growth forms of Trichoderma harzianum

Kalsoom Akhtar, Abdul Tawab, M.Afzal Ghauri…………………………………………………………………………(887)

Antimony accumulation in wild plants around a large Sb deposit area

Zengping Ning, Tangfu Xiao, Yanlong Jia, Fei Yang…………………………………………………………………………(893)

Clay-based microbial bio-composites for chromium (Ⅲ) sorption

Abhilash, Nivedita Singh, B.D.Pandey…………………………………………………………………………(899)

Selective recovery of copper from electroplating wastewater by biogenic sulfide precipitation in a bench-scale batch reactor system

Di Fang, Ruichang Zhang…………………………………………………………………………(904)

Ag⁺ biosorption and silver base nano-particle preparation through Bacillus megaterium growth

Hongxu Li, Binging Liu, Yunchi Guo, Chuanqi Jiao, An Li…………………………………………………………………………(908)

Bacterial adsorption research on the surface of chalcopyrite

Haodan Pan, Hongying Yang, Linlin Tong…………………………………………………………………………(916)

Short Abstracts

Adsorptive removal of Zirconium by Coriolus versicolor

Haq Nawaz Bhatti, Misbah Amin…………………………………………………………………………(921)

Lead biosorption properties of dead biomass of Aspergillus flavus: A local isolate

Kiran Aftab, Kalsoom Akhtar, Abdul Jabbar, M.Afzal Ghauri…………………………………………………………………………(922)

Technology of gold and silver recovery from gold-containing flotation concentrate

Bagdaulet Kenzhaliev, Natalya Zakharoua, Ainur Berkinbayeva, Gulnar Beisembaeyva, Zamzagul Dosymbaeva…………………………………………………………………………(923)

Preparation of novel ionic liquids biosorption material and application on the recovery of metals

Hongmin Cui, Hualing Yang, Wei Wang, Yu Liu, Ji Chen…………………………………………………………………………(924)

Absorption of uranium from solutions by microorganisms

Kh. M.Khamidova, N.K.Beckmukhamedova, N.M.Mukhamedshina, A.A.Mirsagatova…………………………………………………………………………(925)

Roles of metal cations in pellicle formation of Shewanella oneidensis

Yili Liang, Haichun Gao, Xueduan Liu, Guanzhou Qiu…………………………………………………………………………(926)

Removal of Hg(Ⅱ) by biosorption process using Rhodococcus opacus as biosorbent

Jean da Silva Abbud, Antonio Gutirrez Merma, Maurcio Leonardo Torem…………………………………………………………………………(927)

Section VIII: Microbial Ecology

Extended Abstracts

Occurrence of acidophilic halotolerant Fe(Ⅱ)—Oxidizing microorganisms in a marine shore mine tailings deposit at Chaaral, Atacama desert, northern Chile

H.Korehi, D.Kock, B.Dold, A.Schippers…………………………………………………………………………(931)

Molecular assessment of prokaryotes along Upper Ro Agrio (Neuqun, Argentina)

M.Sofa Urbieta, Edgardo Donati, M.Alejandra Giaveno, E.Gonzlez Toril…………………………………………………………………………(936)

Subsurface geomicrobiology of the Iberian Pyritic Belt, preliminary results

Ricardo Amils, David Ferndez-Remolar, Felipe Gmez, Elena Gonzlez-Toril, Nuria Rodriguez,

Jose Luis Sanz, Todd O.Steven and the MARTE Team…………………………………………………………………………(946)

Succession of AMD bacterial communities under high Fe³⁺ concentration

Mengxue Diao, Kai Liu, Minxi Wan, Anh V.Nguyen, Yu Yang…………………………………………………………………………(952)

Evaluation of free-living and particle-attached bacterial population during bioleaching of waste printed circuit boards

Bowei Chen, Zheng Xu, Xingyu Liu, Jiankang Wen…………………………………………………………………………(960)

Geographic distribution of acidophiles around Zijinshan bioheapleaching plant: Dispersal or not?

Xingyu Liu, Renman Ruan, Bowei Chen, Laichang Zou, Gang Zou, Jiankang Wen, Dianzuo Wang

…………………………………………………………………………(964)

Biofilm formation capabilities in the bioleaching microbial consortium: A comparative genomics approach

Harold Nunez, Karina Garces, Juan Pablo Cardenas, Paulo Covarrubias, Amir Shmaryahu, Cecilia Demergasso, David S Holmes, Quatrini Raquel…………………………………………………………………………(970)

Comparative genomics of the oxidative stress response in bioleaching microorganisms

Juan Pablo Cardenas, Paulo Covarrubias, Amir Shmaryahu, Cecilia Demergasso, Gloria Levican, David S Holmes, Raquel Quatrini…………………………………………………………………………(974)

A novel acidophilic γ-proteobacterium for bioleaching

Christian Thyssen, Mario Vera, Claudia Janosch, Wolfgang Sand…………………………………………………………………………(979)

Isolation and characterization of novel Sulfobacillus strains from a commercial bio-heap operation during moderate thermophilic temperature transition

Carol S.Davis-Belmar, D.Cautivo, C.Demergasso, G.Rautenbach…………………………………………………………………………(982)

Diversity of native acid of ile acid drainage from two mining districts of southern ecuador by

16S rDNA sequencing

Yaguana, Leonardo, Aguirre, Paulina y Escobar, Blanca…………………………………………………………………………(988)

Microbial community structure and redox potential in a copper sulphide bioleaching system with low pH, high ferric concentration and elevated temperature

Gang Zou, Renman Ruan, Xingyu Liu, Zengling Wu…………………………………………………………………………(994)

A lusatian open cast lignite mine—A source for new iron-oxidizing microorganisms?

Hans-Michael Siebert, Tobias Uhlig, Silvia Hondke, Wolfgang Sand, K.Peter Stahmann…………………………………………………………………………(1002)

Short Abstracts

Comparison of movement behaviors of Caenorhabdities elegans in response to the treatments in different toxic chemicals at low concentrations

M.G.Mortuza, C.W.Ji, T.-S.Chon…………………………………………………………………………(1007)

Spatial and seasonal variations in microbial populations in a massive, acid drainage generating Pb/Zn mine tailings site

Linan Huang, Wenhua Zhou, Kevin B.Hallberg, Wensheng Shu…………………………………………………………………………(1008)

Study of microbial diversity and key factors affecting community structure on the domesticating progress of the leaching-ore bacterium

Tao Han, Xueduan Liu, Huaqun Yin, Jingxia Zhang, Xing Liu, Yiyao Chen…………………………………………………………………………(1009)

Dissemination and biodiversity of chemolithotrophic bacteria in sulfide ore deposits of armenia

Vardanyan A.K.…………………………………………………………………………(1010)

Microorganisms in reagent free processes of leaching

Zainitdinova L.I., Kukanova S.I., Khuzakulov A.P.…………………………………………………………………………(1011)

Microbial communities in the water body and bottom sediments in natural oil seepage sites Central Baikal

Lomakina A.V., Pavlova O.N., Gorshkov A.G., Morozov I.V., Zemskaya T.I.…………………………………………………………………………(1012)

Microbial community structure and activity in arsenic-rich gold mine bottom sediments

Lukasz Drewniak, Aleksandra Sklodowska…………………………………………………………………………(1013)

The ecophysiology of bioleaching: Who does what to whom and when

Raquel Quatrini, Cecilia Demergasso, Eugenia Jedlicki, David S.Holmes…………………………………………………………………………(1014)

Identification and analysis of diazotrophy in strains of Leptospirillum ferriphilum from heap bioleaching operations

Galleguillos P.A., Demergasso C.S., Johnson D.B., Quatrini R., Holmes D.S., Hallberg K.B.…………………………………………………………………………(1015)

Characterisation of novel oxalate-degrading beta-Proteobacteria isolated from a full-scale bioreactor treating Bayer liquor organic waste

McSweeney N.J., Franzmann P.D., Tilbury A.L., Nyeboer H.J., McKinnon A.J., Sutton D.C., Kaksonen A.H.…………………………………………………………………………(1016)

Iron reducing bacteria can be aerobic?

Abhilash, Shantoshini Dash, B.D.Pandey…………………………………………………………………………(1017)

Impact of minerology and environmental factors on the acidophiles distribution in three uranium deposits

Yajie Liu, Jiang Li, Lingling Xu, Weiyun Xu, Zhanxue Sun, Jinhui Liu, Xueli Li, Weijun Shi…………………………………………………………………………(1018)

Acidophilic community succession in uranium column bioleaching

Yajie Liu, Jiang Li, Lingling Xu, Weiyun Xu, Jinhui Liu, Xueli Li, Weijun Shi, Jianshe Liu…………………………………………………………………………(1019)

Fungal diversity and metal ion resistance in an extreme acidic environment, the riotinto

Oggerin, Monike, Del Moral, Catalina, Rodriguez, Nuria, Amils, Ricardo…………………………………………………………………………(1020)

Review of the microbial ecology of BIOX reactors illustrates the dominance of the genus Ferroplasma in many commercial reactors

Robert P.van Hille, Nathan van Wyk, Susan T.L.Harrison…………………………………………………………………………(1021)

Biosynthesis of sterols and wax esters by euglena of acid mine drainage biofilms: Implications for eukaryotic evolution and the early earth

Jiasong Fang, Shamik Das Gupta, Sandra S.Brake, Stephen T.Hasiotis, Li Zhang…………………………………………………………………………(1022)

Section IX: Mining Impacts on the Environment

Extended Abstracts

Treatment and reuse of complex copper-lead-zinc dressing wastewarter

Daixiong Chen, Bo Hu, Tong Chen, Jianwen Yang, Lin Luo, Wei Xue…………………………………………………………………………(1025)

An environment-friendly leaching process of converter vanadium-bearing slag

Wangzhong Mu, Ting’an Zhang, Zhihe Dou, Guozhi L, Yan Liu…………………………………………………………………………(1031)

Current situation and outlook on biological treatment of wastewater in metallic mine

Siqing Liu, Cao Ye, Xiong Tong, Luzhen Chen…………………………………………………………………………(1037)

Microorganisms of tailing dumps of Uzbekistan and their role in cyanides destruction

Kukanova S.I., Pulatova O.M., Zaynitdinova L.I., Akin’shina G.I.…………………………………………………………………………(1044)

Unascertained classify optimization method to evaluate destructive effect of masonry structure under blasting vibration of open-pit mine

Longjun Dong, Xibing Li…………………………………………………………………………(1047)

Study on recovery of iron containing in reverse-flotation tailings of iron ore

Shuling Gao, Dezhou Wei, Wengang Liu, Zichuan Guan…………………………………………………………………………(1053)

Short Abstracts

The impact of Artisanal gold mining and the effort to minimize the ngative impact to the environment

H.Husaini, Lili Tahli…………………………………………………………………………(1059)

The impact of bauxite washing and the effort to minimize the negative impact to the environment

H.Husaini, Lili Tahli…………………………………………………………………………(1060)

Synthesis of CaO nanoparticles by microwave to treat heavy metal

Arup Roy, Jayanta Bhattacharya…………………………………………………………………………(1061)

Effect of iron minerals on the stability of microbially immobilized zinc

Yamini Satyawali, Winnie Dejonghe, Sandra Van Roy, Ludo Diels, Karolien Vanbroekhoven…………………………………………………………………………(1062)

Acid base accounting and kinetic leaching test application for appraisal of acid drainage in Raniganj Coalfield, India

Dibyendu Banerjee…………………………………………………………………………(1063)

Section X: Bioremediation of Metals and Mine Wastes

Extended Abstracts

Treatment of heavily polluted acid mine drainage by means of a multi-component passive system

Stoyan Groudev, Irena Spasova, Marina Nicolova, Plamen Georgiev…………………………………………………………………………(1067)

Phytoremediation experiments at a former uranium mining site, and subsequent utilization of the plant residue

S.Willscher, D.Mirgorodsky, L.Jablonski, D.Ollivier, D.Merten, G.Bchel, J.Wittig, P.Werner…………………………………………………………………………(1073)

Sulphate-reducing fixed-bed bioreactors fed with different organic substrates for metal precipitation

Pavlina Kousi, Emmanouella Remoundaki, Artin Hatzikioseyian, Marios Tsezos…………………………………………………………………………(1078)

The recovery of platinum group metals from model solutions and fabrication into new catalysts

Scott Taylor, Angela J.Murray, Stuart Blackburn, Lynne E.Macaskie…………………………………………………………………………(1085)

Microbial reduction of chromite overburden for recovery of Nickel and Cobalt: An alternative of thermal reduction

N.Pradhan, R.R.Nayak, E.Priyadarshini, L.B.Sukla, B.K.Mishra…………………………………………………………………………(1090)

Bioremediation of waste water sludge from heavy metals

Levon Markosyan, Narine Vardanyan…………………………………………………………………………(1096)

Immobilization of arsenite from the refining discharge using Acidianus brierleyi in the presence of pyrite

Keiko Sasaki, Masaharu Koga, Koichiro Takatsugi, Naoko Okibe, Tsuyoshi Hirajima, Satoshi Asano, Shinichi Heguri…………………………………………………………………………(1106)

Bioremediation of Cr(Ⅵ) from chromite mine drainage using native fungal isolates

B.Dhal, K.D.Mehta, B.D.Pandey, N.N.Das, H.N.Thatoi…………………………………………………………………………(1109)

Research and application of biological oxidation metal refining craft under extreme environment

Fulai Hao, Guping Zhang, Xiumei Wang, Jinchang Gao…………………………………………………………………………(1115)

Bioleaching of heavy metals from contaminated alkaline sediment by auto- and heterotrophic bacteria in a stirred tank reactor

Jianyu Zhu, Jingxia Zhang, Qian Li, Tao Han, Yuehua Hu, Xueduan Liu, Wenqing Qin, Liyuan Chai, Guanzhou Qiu…………………………………………………………………………(1122)

Short Abstracts

Release of valuable metals from various solid wastes by bioleaching technology and mechanisms

Baoping Xin, Wenfeng Jiang, Di Zhang…………………………………………………………………………(1128)

Selective recovery of transition metals from mine waters by novel consortia of acidophilic sulfate reducing bacteria

Ivan Nancucheo, D.Barrie Johnson…………………………………………………………………………(1129)

Commissioning and demonstration of continuous flow reactors for the selective bio-oxidation and precipitation of iron in mine waters

Sabrina Hedrich, D.Barrie Johnson…………………………………………………………………………(1130)

Sweet meat waste as organic carbon source for biological sulfate reduction

Bidus Kanti Das, Jayanta Bhattacharya…………………………………………………………………………(1131)

Effects of carbon drought on metals and sulfate immobilization in a chemo-bioreactor treating acid mine drainage

Jayanta Bhattacharya, Bidus Kanti Das…………………………………………………………………………(1132)

Arsenic (As)-resistant bacteria in S-As-rich acid mine minerals of West Java Province, Indonesia: Implications for bioremediation of As-polluted areas

Siti Khodijah Chaerun, Intan Nurul Rizki…………………………………………………………………………(1133)

Biodegradation of xanthate using Pseudomonas putida

M.R.Sabari Prakasan, K.A.Natarajan…………………………………………………………………………(1134)

Stirred tank bioleaching experiments of solid waste materials from copper, steel and recycling industries

Vestola E.A., Kuusenaho M.K., Nrhi H.M., Tuovinen O.H., Puhakka J.A., Plumb J.J., Kaksonen A.H.…………………………………………………………………………(1135)

Effect of Fe(Ⅲ) on the biotreatment of bioleaching solutions containing heavy metals of low concentration using sulfate-reducing bacteria

Junya Cao, Guangji Zhang, Zaisha Mao, Zhaoheng Fang, Chao Yang…………………………………………………………………………(1136)

Bio-characteristics and treating acid mine wastewater of a sulfate reducing bacterium

Wenyan Liu, Bowei Chen, Minglin Wu, Jiankang Wen…………………………………………………………………………(1137)

Author Index…………………………………………………………………………(1139)

Keyword Index…………………………………………………………………………(1147)